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Title:Wide Pick Filament from Pick's disease brain
Authors:Falcon B, Zhang W, Murzin AG, Murshudov G, Garringer HJ, Vidal R, Crowther RA, Ghetti B, Scheres SHW, Goedert M
Sample:microtubule-associated protein tau
Method:Helical reconstruction (8.0 angstroms resolution)
Red flagLatest update:2018-09-19
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Sample
Sample name: microtubule-associated protein tau
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1cellular-componentmicrotubule-associated protein taufalse
2proteinWide Pick Filament from Pick's disease brainfalse
Experiment
Specimen state: Tissue
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.40.5 mg/mL
Helical parameters:
HandPhi incrementAzimuthal (Z) incrementRotational symmetry
RIGHT HANDED-0.6°4.7 ÅC2
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%277 KFEI VITROBOT MARK IV ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS300 kVFLOOD BEAMBRIGHT FIELD2.7 mm1700.0 nm2800.0 nmFIELD EMISSION GUNGATAN K2 QUANTUM (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
OTHER°°GIF Quantum LS eV K K K mrad1.06 e/Å2
Processing
Resolution by author:8.0 Å
Resolution method:FSC 0.143 CUT-OFF
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails