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Title:Asymmetric region of the Membrane Attack Complex in the closed conformation
Authors:Menny A, Serna M, Boyd CB, Gardener S, Joseph AP, Topf M, Bubeck D
Sample:Membrane Attack Complex
Method:Single particle reconstruction (5.9 angstroms resolution)
Red flagLatest update:2018-12-26
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Sample
Sample name: Membrane Attack Complex
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinMembrane Attack ComplexfalseHomo sapiensProtein complex was assembled on liposomes and detergent solubilized
2proteinC5bfalseHomo sapiensComponent of the Membrane Attack Complex
3proteinC6falseHomo sapiensComponent of the Membrane Attack Complex
4proteinC7falseHomo sapiensComponent of the Membrane Attack Complex
5proteinC8 alphafalseHomo sapiensComponent of the Membrane Attack Complex
6proteinC8 betafalseHomo sapiensComponent of the Membrane Attack Complex
7proteinC8 gammafalseHomo sapiensComponent of the Membrane Attack Complex
8proteinC9falseHomo sapiensComponent of the Membrane Attack Complex
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.4 mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TITAN KRIOS300 kVSPOT SCANBRIGHT FIELD mm nm nm59000.0FIELD EMISSION GUNFEI FALCON II (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
OTHER°° eV K K K mrad50.0 e/Å2
Processing
Software:RELION
Number of particles:53167
Imposed symmetry:C1
Resolution by author:5.9 Å
Resolution method:FSC 0.143 CUT-OFF
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
13009 μm/pixel
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails