The reconstruction was done by aligning the members of
the tilt series using crosscorrelation functions, such as
phase only and the VanHeel Mutual Correlation Function.
The sampling in each micrographof a tilted specimen was
match by interpolation to th e lower angle members of the
tilt series. This matching of areas and sampling was done
by a 4 parameter grid search. Once aligned and scaled, the
data were combined in Fourier space using a
Whittaker-Shannon interpolation scheme. The map was calculated
using a reverse Fourier transformation. The software used for
the reconstruction was in-house and adapted from in some
cases from MRC software.
poor atom-atom contacts and cross correlation coefficient
RSref
REAL
Domains were fit manually into the density using O.
The entire light chain domain consisting of heavy chain residues
from G710 to K843 and the two light chains were repositioned
using G710 of the myosin heavy chain using O.Real space
refinement done using 7 rigid bodies and 4 hinge points.
