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Title:Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex.
Authors:H. Stark, M.V. Rodnina, H.J. Wieden, F. Zemlin, W. Wintermeyer, M. van Heel
Sample:Kirromycin stalled 70S Ribosome from E.coli
Aggregation state:Single particle (13 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: Kirromycin stalled 70S Ribosome from E.coli
Oligomeric state: monomer
Theoretical molecular weight of the sample: 2.5
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-prokaryote70S Ribosome
2ligandEF-Tu, tRNA, GTPfalse
3nucleic-acidformyl-methionyl tRNAfalse
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.50.07 mg/mL50mM Tris-HCl, 70mM NH4Cl, 30mM KCl, 7mM MgCl2, 1mM DTT
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE40% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/SOPHIE120 kVFLOOD BEAMBRIGHT FIELD1.35 mm800 nm2500 nm58500FIELD EMISSION GUNKodak SO163 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side entryHOME BUILD°° eV4.2 K4.2 K4.2 K mrad15 e/Å2
Processing
Software:Imagic
CTF correction:phase flip
Resolution by author:13 Å
Resolution method:FSC at 3 sigma
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
13.2 μm/pixel1.38linkPATCHWORK DENSITOMETER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1MJ1 rigid bodySitus and AmiraThe domains of EF-Tu were fitted seperately by visual docking using O and Swiss PDB viewer
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