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Title:A cryo-electron microscopic study of ribosome-bound termination factor RF2.
Authors:Urmila Rawat, A.V. Zavialov,J. Sengupta, M. Valle, R.A. Grassucci, J. Linde, B. Vestergaard, M. Ehrenberg, J. Frank
Sample:RF2 (GAQ) mutant from E.coli
Aggregation state:Single particle
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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bullet_white.gif  Downloads
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Sample
Sample name: RF2 (GAQ) mutant from E.coli
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1ligandRF2 (GAQ) mutanttrue
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD mm nm nmFIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Oxford, cryo-transfer 3500GATAN LIQUID NITROGEN°° eV K K K mrad e/Å2information is the same as in submission 1186
Processing
Processing details:information is the same as in submission 1186. SPIDER was used for the computational isolation of the RF2 (GAQ) mutant cryo-density from the 3D-EM map of RF2 mutant bound to the E.coli 70s ribosome (submission 1186).
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixellinkinformation is the same as in submission 1186ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1M16 1MVR 
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