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Title:The DnaB.DnaC complex: a structure based on dimers assembled around an occluded channel.
Authors:M.Barcena,T.Ruiz,L.E.Donate,S.E.Brown,N.E.Dixon,M.Radermacher,J.M.Carazo
Sample:DnaB.DnaC complex from Escherichia coli
Aggregation state:Single particle (26 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: DnaB.DnaC complex from Escherichia coli
Oligomeric state: one homohexamer of DnaB binds to six monomers of DnaC
Theoretical molecular weight of the sample: 0.48
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinDnaBhexamertrueEscherichia coliGO:0003678IPR001198
2proteinDnaCthree dimerstrueEscherichia coli
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.60.03 mg/mL50 mM Tris-HCl, 25 mM NaCl 5 mM MgCl2 2 mM DTT 0.1 mM ATP
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGERdouble-blotting msVitrification instrument: plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM120T100 kVFLOOD BEAMBRIGHT FIELD2.2 mm1000 nm2000 nm6000057874LAB6kodak S0163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
cryo-holderGATAN LIQUID NITROGEN0°35° eV K K K mrad e/Å2
Processing
Software:SPIDER and Xmipp
Resolution by author:26 Å
Resolution method:FSC at a 5 times FSC with noise cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
587 μm/pixel8linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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