top_cornerHomeEMD-1019  Contact us 
Image unavailable
Title:The Escherichia coli large ribosomal subunit at 7.5 A resolution.
Authors:R.Matadeen
Sample:50S E.coli ribosomal subunit
Aggregation state:Single particle (7.5 angstroms resolution)
Red flagLatest update:2011-05-26
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: 50S E.coli ribosomal subunit
Oligomeric state: single entity
Theoretical molecular weight of the sample: 1.5
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-prokaryote50S ribosomal subunit1.5
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.41 mg/mL20mM HEPES-KOH 6 mM MgCl2, 150 mM NH4Cl
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGERBlot for 2 seconds before plunging msVitrification instrument: in-house freeze plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/UT200 kVFLOOD BEAMBRIGHT FIELD2 mm1000 nm3000 nm3800037600FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
eucentricGATAN LIQUID NITROGEN°° eV96 K K K mrad10 e/Å2
Processing
Software:Imagic
CTF correction:Each particle
Resolution by author:7.5 Å
Resolution method:FSC at 3 sigma cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
75 μm/pixel16linkPATCHWORK DENSITOMETER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
ESSENS
outer_corner