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Title:Structure of a viral DNA gatekeeper at 10 A resolution by cryo-electron microscopy.
Authors:E.V.Orlova, B.Gowen, A.Droege, A. Stiege, F.Weise, R.Lurz, M. van Heel, P. Tavares
Sample:Bacteriophage SPP1 portal protein gp6
Aggregation state:Single particle (10 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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Sample
Sample name: Bacteriophage SPP1 portal protein gp6
Oligomeric state: isolated portal protein has 13- fold cyclical symmetry
Theoretical molecular weight of the sample: 0.0573
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinBacteriophage SPP1 portal protein gp6C13trueBacilus subtilis Bacteriophage SPP1
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.51.5 mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGERBlot about 2-3 seconds before plunging. msVitrification instrument: Standard plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/ST200 kVFLOOD BEAMBRIGHT FIELD2.1 mm1700 nm3000 nm5000048600FIELD EMISSION GUNKodak SO163 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side-entryGATAN LIQUID NITROGEN°° eV K K K mrad10 e/Å21999-01-01
Processing
Software:IMAGIC
CTF correction:CTF correction of each particle
Resolution by author:10 Å
Resolution method:FSC at 20 sigma
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1510.2 μm/pixel1.68linkImages were digitised using a patch work densitometer.PATCHWORK DENSITOMETER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
Angular reconstitutionMolrep
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