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Title:Structure of a viral DNA gatekeeper at 10 A resolution by cryo-electron microscopy.
Authors:E.V. Orlova, B. Gowen, A. Droege, A. Stiege, F. Weise, R. Lurz, M. van Heel, P. Tavares
Sample:Bacteriophage SPP1 connector
Aggregation state:Single particle (10 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: Bacteriophage SPP1 connector
Oligomeric state: cyclical dodecamer
Theoretical molecular weight of the sample: 0.96
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinBacteriophage SPP1 portal protein gp60.0573cyclical dodecamerfalseBacillus subtilis bacteriophage SPP1
2proteingp150.0116cyclical dodecamerfalseBacillus subtilis bacteriophage SPP1
3proteinhead completion protein gp1612.5cyclical dodecamerfalseBacillus subtilis bacteriophage SPP1
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.50.9 mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGERblot for 2-3 second before plunging msVitrification instrument: general plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/ST200 kVFLOOD BEAMBRIGHT FIELD2.1 mm1700 nm3000 nm5000048600FIELD EMISSION GUNKodak SO163 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side-entryGATAN LIQUID NITROGEN°° eV90 K K K mrad10 e/Å21999-01-01
Processing
Software:Imagic
CTF correction:Each particle
Resolution by author:10 Å
Resolution method:FSC at 20 sigma
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
2110.2 μm/pixel1.88linkPATCHWORK DENSITOMETER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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