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Title:Six molecules of SV40 large T antigen assemble in a propeller-shaped particle around a channel.
Authors:C. San Martin
Sample:SV40 T antigen
Aggregation state:Single particle (29 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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Sample
Sample name: SV40 T antigen
Oligomeric state: homohexamer
Theoretical molecular weight of the sample: 0.5
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinLarge T antigenhomohexamertrueSimian virus 40GO:0003688IPR003133
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.5.06 mg/mL20 mM Tris.HCl pH 7.5 5 mM KCl 1.5 mM MgCl2 0.1 mM DTT 4 mM ADP2% Uranyl Acetate
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 1200EXII120 kVFLOOD BEAMBRIGHT FIELD5.6 mm nm nm60000TUNGSTEN HAIRPINKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
JeolJEOL°55° eV K K K mrad10 e/Å2
Processing
Software:Xmipp
Resolution by author:29 Å
Resolution method:DPR at 45 degrees
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
8 μm/pixel8linkEIKONIX IEEE 488
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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