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Title:Image reconstructions of microtubules decorated with monomeric and dimeric kinesins: comparison with x-ray structure and implications for motility.
Authors:A. Hoenger
Sample:Rat kinesin motor domain complexed to microtubules without nucleotides
Aggregation state:Helical (25 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: Rat kinesin motor domain complexed to microtubules without nucleotides
Oligomeric state: dimer
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinrat kinesindimertrueRattus norvegicus
2proteintubulinhetero dimertrueRattus norvegicus
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.80.5 mg/mLPipes 80mM, MgCl 1mM, GTP 1mM, Taxol 20uM, DMSO 5%ice-embeddedholey grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%93 KHOMEMADE PLUNGER msVitrification instrument: self made
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
OTHER100 kVFLOOD BEAMBRIGHT FIELD mm1000 nm1600 nm35000LAB6AGFA Scienta Film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side-entryGATAN LIQUID NITROGEN0°0° eV95 K K K mrad5 e/Å2
Processing
Software:Phoelix, Suprim
Resolution by author:25 Å
Resolution method:FSC at 0.5
Processing details:Final maps from 20 averaged datasets = 10 helical tubes
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1020 μm/pixel16linkEMIL 10
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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