| 7.9 | 0.05 mg/mL | 20 mM Hepes, 600 mM KCl, 1.5 mM Mg2 Cl2, 5% glycerol,
0.5 mM DTT, 0.5 mM PMSF | The SF3b complexes were sandwiched in 2% uranyl formate
between two layers of thin carbon using a perforated carbon grid
as support. After 2 min at RT, the sample was frozen in liquid nitrogen. | 200 mesh copper grid |
