top_cornerHomeEMD-1047  Contact us 
Image unavailable
Title:ATP-bound states of GroEL captured by cryo-electron microscopy.
Authors:H.R. Saibil
Sample:GroEL-ATP from E.coli
Aggregation state:Single particle (14.9 angstroms resolution)
Red flagLatest update:2011-05-26
inner_corner
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: GroEL-ATP from E.coli
Oligomeric state: 14-mer
Theoretical molecular weight of the sample: 0.8
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinGroEL0.814-mertrueEscherichia coliD398A mutant; The Asp398Ala mutant of GroEL has severely reduced ATPase activity but can support a round of protein folding.
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.50.8 mg/mL12.5 mM HEPES, 5 mM KCl, 5 mM MgCl2, 250 microM ATPholey carbon film
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%100 KHOMEMADE PLUNGERBlot for 1 second before plunging msVitrification instrument: self made
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG/ST200 kVFLOOD BEAMBRIGHT FIELD2 mm1200 nm5000 nm3800036080FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV105 K K K mrad20 e/Å2
Processing
Software:Spider
CTF correction:CTF multiplication and merging of 2D averages
Resolution by author:14.9 Å
Resolution method:FSC at 0.5
Processing details:Filtered back projection
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1607 μm/pixel18linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1DER Rigid bodyDockEMREALManual fitting of the 3 domains of a subunit as rigid bodies using O, followed by refinement with DockEM
outer_corner