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Title: Three-dimensional structure of bacteriophage T4 baseplate. Authors: Kostyuchenko VA, Leiman PG, Chipman PR, Kanamaru S, vanRaaij MJ, Arisaka F, Mesyanzhinov VV, Rossmann MG Sample: Bacteriophage T4 baseplate-tail tube assembly Method: Single particle reconstruction (12.0 angstroms resolution) Latest update: 2012-10-24   Summary  Experimental details   Visualization   Map information   Downloads Sample Sample name: Bacteriophage T4 baseplate-tail tube assembly Oligomeric state: the complex has sixfold symmetry Components: ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details 1 protein baseplate-tail tube complex true Enterobacteria phage T4 Experiment Specimen state: Particle Specimen preparation: pH Specimen conc. Details Staining Specimen support details 7 5 mg/mL water holey carbon Vitrification: Cryogen name Humidity Temp. Instr. Method Time resolved Details ETHANE % K Blot for 2 seconds before plunging ms Imaging: Microscope Voltage Illumination mode Imaging mode Cs Defocus min. Defocus max. Nominal mag. Calibrated mag. Electron source Detector Detector distance Astigmatism FEI/PHILIPS CM300FEG/T 300 kV FLOOD BEAM BRIGHT FIELD 2 mm 1200 nm 5000 nm 45000 47000 FIELD EMISSION GUN Kodak SO163 film mm Specimen holder Holder model Tilt min. Tilt max. Energy filter Energy window Temp. Temp. min. Temp. max. Beam tilt Electron dose Other details Date 626 Single Tilt Cryotransfer System GATAN LIQUID NITROGEN 0° 0° eV K K K mrad 25 e/Å2 2001-01-30 Processing Protocol: model based projection matching Software: SPIDER CTF correction: each particle Number of particles: 945 Resolution by author: 12.0 Å Resolution method: FSC at 0.5 cut-off Processing details: Modified SPIDER version was used to allow reconstruction of the whole baseplate-tail tube assembly. Scanned images: Num. images Sampling size OD range Quant. bit number Other details Scanner 15 14 μm/pixel 8 images were scanned at 7 micron per pixel and averaged 2x2 to give 14 micron per pixel ZEISS SCAI Fitting: PDB Protocol Target crit. Software B value Fitting space PDB chain Details rigid body correlation coefficient Situs 2.0, Colores REAL Protocol: laplacian filtered real space rigid body correlation coefficient Situs 2.0, Colores REAL Protocol: laplacian filtered real space rigid body correlation coefficient Situs 2.0, Colores REAL Protocol: laplacian filtered real space rigid body correlation coefficient Situs 2.0, Colores REAL Protocol: laplacian filtered real space rigid body correlation coefficient Situs 2.0, Colores REAL Protocol: laplacian filtered real space rigid body correlation coefficient Situs 2.0, Colores REAL Protocol: laplacian filtered real space rigid body correlation coefficient Situs 2.0, Colores REAL Protocol: laplacian filtered real space rigid body correlation coefficient Situs 2.0, Colores REAL Protocol: laplacian filtered real space