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Title:A cellular receptor of human rhinovirus type 2, the very-low-density lipoprotein receptor, binds to two neighboring proteins of the viral capsid.
Authors:E. Neumann, R. Moser, L.Snyers, D. Blaas and E.A. Hewat
Sample:Human Rhinovirus 2 in complex with its cellular receptor, VLDL-R
Aggregation state:Icosahedral (16 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: Human Rhinovirus 2 in complex with its cellular receptor, VLDL-R
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusHuman rhinovirus 2Human rhinovirus 2
2cellular-componentCellular ReceptortrueunidentifiedThe VLDL-R consists of eight imperfect ligand-binding repeats of approximately 40 amino acids at its N-terminus. Recombinant VLDL-minireceptors encompassing different ligand-binding repeats are here expressed with the first three repeats + a Maltose Binding Protein fused to the N-terminus + a Hexa-his-tag fused to the C-terminus.
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.40.82 mg/mL50 mM Tris-HCl for the virus 2 mM CaCl2, 20 mM Tris-HCL for the receptor fragment300 mesh copper grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KBlot with filter paper for 1-2 seconds ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2010F200 kVSPOT SCANBRIGHT FIELD1.4 mm1180 nm2900 nm4000039700FIELD EMISSION GUNKodak SO163 film mmobjective lens astigmatism was corrected at 300,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Oxford cryo-holder CT3200OTHER°° eV K K K mrad15 e/Å2
Processing
CTF correction:CTFMIX
Resolution by author:16 Å
Resolution method:FSC at 0.5 cut-off
Processing details:Methods for reconstructing density maps of "single" particles from cryoelectron micrographs to subnanometer resolution (Conway et al.,J Struct Biol. 1999 Dec 1;128(1):106-18.)
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
337 μm/pixellinkZeiss PhotoScan TD scannerOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
Manual dockingOREALFitting the X-RAY structures of HRV2 and the VLDL-R repeats to the cryo-electron microscope reconstructed density. Determination of the residues included in the footprints.
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