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Title:Structure of the signal recognition particle interacting with the elongation-arrested ribosome.
Authors:M Halic, R Beckmann
Sample:80S RNC-SRP complex fron canis sp.
Aggregation state:Single particle (12.0 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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Sample
Sample name: 80S RNC-SRP complex fron canis sp.
Oligomeric state: one srp binds one ribosome
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-eukaryote80S ribosome4q
2ligandmammalian SRP0.4monomertrueq
3ligandtRNA0.025monomertrueq
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F30300 kVFLOOD BEAMBRIGHT FIELD2 mm1000 nm4300 nmFIELD EMISSION GUNKODAK SO163 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV K K K mrad10 e/Å2
Processing
Software:spider
Resolution by author:12.0 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
μm/pixellinkOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1RY1 The domains were separately fitted by manual docking using program O
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