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Title:Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.
Authors:C.M.Spahn, J.Frank.
Sample:Ribosomal 80S-eEF2-sordarin complex from S. cerevisiae
Aggregation state:Single particle (11.7 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: Ribosomal 80S-eEF2-sordarin complex from S. cerevisiae
Theoretical molecular weight of the sample: 3.2
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-eukaryote80S ribosome
2proteineEF2falseunidentified
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm nm nm5000052000FIELD EMISSION GUNKodak SO163 film mm100,000 X magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Oxford, cryo-transfer 3500GATAN LIQUID NITROGEN0°0° eV93 K K K mrad15 e/Å2
Processing
Software:SPIDER
CTF correction:defocus group volumes
Resolution by author:11.7 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
86 μm/pixel12linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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