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| Title: | Three-dimensional structures of translating ribosomes by Cryo-EM. |
| Authors: | R.J.C.Gilbert, P.Fucini, S.Connell, S.D.Fuller, K.H.Nierhaus, C.V.Robinson, C.M.Dobson, D.I.Stuart |
| Sample: | E. coli ribosome translating GFP |
| Aggregation state: | Single particle (16 angstroms resolution) |
 Latest update: | 2011-05-26 |
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| Processing |
| Software: | SPIDER, IMAGIC, GAP, CNS, XPLOR |
| CTF correction: | Each negative dataset |
| Resolution by author: | 16 Å
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| Resolution method: | FSC at 0.5 cut-off |
| Processing details: | Final maps were calculated from 2276 images from
a total of 4500 from 10 individual datasets,
with scaling in reciprocal space to
crystallographic ribosome structures and
correction for map anisotropy by
B-factor weighting of amplitudes in
XPLOR with respect to a similarly-treated
control inactive ribosome. Selection of
particles for inclusion in the final
maps was by correlation coefficient with respect
to the alignment model, designed to maximise nascent
chain occupancy in the selected images. |
| Unit cell: |
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| Scanned images: |
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| Fitting: |
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