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| Title: | Three-dimensional structures of translating ribosomes by Cryo-EM. |
| Authors: | R.J.C.Gilbert, P.Fucini, S.Connell, S.D.Fuller, K.H.Nierhaus, C.V.Robinson, C.M.Dobson, D.I.Stuart |
| Sample: | E. coli ribosome translating GFP and tandem Ig domains -
difference map |
| Aggregation state: | Single particle (16 angstroms resolution) |
 Latest update: | 2011-05-26 |
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| Processing |
| Software: | SPIDER, IMAGIC, GAP, CNS, XPLOR |
| CTF correction: | Each negative dataset |
| Resolution by author: | 16 Å
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| Resolution method: | FSC at 0.5 cut-off |
| Processing details: | See entries 1068, 1070, 1071 and 1072. Vector difference
maps were calculated between reconstructions of each nascent
protein-ribosome complex and the control inactive map. The
difference densities for the tandem Ig domains and GFP nascent
proteins were more similar to each other than either was to the
single Ig domain nascent protein. Therefore these two difference
maps were averaged together to produce a set of consensus
difference densities representing the main differences between
inactive ribosomes and ribosomes stalled during translation that
contain a nascent polypeptide chain. |
| Unit cell: |
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| Scanned images: |
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| Fitting: |
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