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Title:A 11.5 A single particle reconstruction of GroEL using EMAN.
Authors:S.J. Ludtke, J. Jakana, J. Song, D.T. Chuang, W. Chiu
Sample:native naked GroEL
Aggregation state:Single particle (11.5 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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Sample
Sample name: native naked GroEL
Oligomeric state: homo 14-mer
Theoretical molecular weight of the sample: 0.8
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinGroEL0.05714-mertrueEscherichia coli
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
1.0 mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER msVitrification instrument: Manual, gravity, plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 4000EX400 kVFLOOD BEAMBRIGHT FIELD4.1 mm1000 nm2400 nm50000LAB6SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entryGATAN LIQUID NITROGEN°° eV K K K mrad30 e/Å2
Processing
Software:EMAN
CTF correction:Per particle phase flipping, amplitude correction during averaging
Resolution by author:11.5 Å
Resolution method:FSC at 0.5
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
57 μm/pixel8linkData was median filtered to 14 microns/pixel after scanning.ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1OEL Rigid Bodycorrelation coefficientfoldhunter EMANREAL
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