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Title:A 11.5 A single particle reconstruction of GroEL using EMAN.
Authors:Ludtke SJ, Jakana J, Song J, Chuang DT, Chiu W
Sample:native naked GroEL
Method:Single particle reconstruction (11.5 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: native naked GroEL
Oligomeric state: homo 14-mer
Theoretical molecular weight of the sample: 0.8
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinGroEL0.05714-mertrueEscherichia coli
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
1.0 mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER msVitrification instrument: Manual, gravity, plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 4000EX400 kVFLOOD BEAMBRIGHT FIELD4.1 mm1000 nm2400 nm50000LAB6KODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entryGATAN LIQUID NITROGEN°° eV K K K mrad30 e/Å2
Processing
Software:EMAN
CTF correction:Per particle phase flipping, amplitude correction during averaging
Number of particles:4916
Number of class averages:334
Imposed symmetry:D7
Resolution by author:11.5 Å
Resolution method:FSC 0.5
Processing details:Note that class averages are EMAN-style reference-based, not MSA.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
57 μm/pixel8Data was median filtered to 14 microns/pixel after scanning.ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1OEL rigid bodycorrelation coefficientfoldhunter EMANREALProtocol: Rigid Body