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Title:Seeing GroEL at 6 A resolution by single particle electron cryomicroscopy.
Authors:S.J. Ludtke, D. Chen, J. Song, D.T. Chuang,W. Chiu
Sample:native naked GroEL
Aggregation state:Single particle (6 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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Sample
Sample name: native naked GroEL
Oligomeric state: homo 14-mer
Theoretical molecular weight of the sample: 0.8
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinGroEL0.05714mertrueEscherichia coli
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
1.0 mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER msVitrification instrument: Manual gravity plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2010F200 kVFLOOD BEAMBRIGHT FIELD1.0 mm794 nm2560 nm50000FIELD EMISSION GUNSO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entryGATAN LIQUID NITROGEN°° eV K K K mrad18 e/Å2
Processing
Software:EMAN
CTF correction:per-particle phase flipping pre-processing, amplitude correction during class averaging
Resolution by author:6 Å
Resolution method:FSC at 0.5
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
427 μm/pixel8linkData was median filtered to 10.5 microns/pixel after scanning.ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1OEL Rigid Bodycorrelation coefficientfoldhunter, EMANREALrigid body fitting of 1 monomer from 1OEL initially, followed by rigid body refinement of each domain, apical, intermediate and equatorial.
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