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| Title: | The PM2 virion has a novel organization with an internal membrane and pentameric receptor binding spikes. |
| Authors: | J.T. Huiskonen, H.M. Kivela, D.H. Bamford, S.J. Butcher |
| Sample: | Proteinase K-treated PM2 virus |
| Aggregation state: | Icosahedral (13 angstroms resolution) |
 Latest update: | 2011-05-26 |
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| Experiment |
| Sample preparation: |
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| Vitrification: |
| Cryogen name | Humidity | Temp. | Instr. | Method | Time resolved | Details |
| ETHANE | % | 90 K | HOMEMADE PLUNGER | A small vial of ethane is placed inside a larger
liquid nitrogen reservoir. The grid holding 3
microliters of the sample is held in place at the bottom
of a plunger by the means of fine tweezers. When the
liquid ethane is ready, a piece of filter paper is then
pressed against the sample to blot off excess buffer,
sufficient to leave a thin layer on the grid. The filter paper
is removed, and the plunger is allowed to drop into the
liquid ethane. Once the grid enters the liquid ethane,
the sample is rapidly frozen, and the grid is
transferred under liquid nitrogen to a storage box
immersed in liquid nitrogen for later use in the microscope. | ms | Vitrification instrument: EMBL design |
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| Imaging: |
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