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| Title: | The PM2 virion has a novel organization with an internal membrane and pentameric receptor binding spikes. |
| Authors: | J.T. Huiskonen, H.M. Kivela, D.H. Bamford, S.J. Butcher |
| Sample: | Bromelain-treated PM2 virus |
| Aggregation state: | Icosahedral (13.4 angstroms resolution) |
 Latest update: | 2011-05-26 |
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| Experiment |
| Sample preparation: |
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| Vitrification: |
| Cryogen name | Humidity | Temp. | Instr. | Method | Time resolved | Details |
| ETHANE | % | 90 K | HOMEMADE PLUNGER | A small vial of ethane is placed inside a larger
liquid nitrogen reservoir. The grid holding 3
microliters of the sample is held in place at the
bottom of a plunger by the means of fine tweezers.
When the liquid ethane is ready, a piece of
filter paper is then pressed against
the sample to blot off excess buffer,
sufficient to leave a thin layer on the grid. The
filter paper is removed, and the plunger is
allowed to drop into the liquid ethane. Once
the grid enters the liquid ethane, the sample is
rapidly frozen, and the grid is transferred under liquid
nitrogen to a storage box immersed in liquid
nitrogen for later use in the microscope. | ms | Vitrification instrument: EMBL design |
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| Imaging: |
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