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Title:Nuclear pore complex structure and dynamics revealed by cryoelectron tomography.
Authors:Beck M, Forster F, Ecke M, Plitzko JM, Melchior F, Gerisch G, Baumeister W, Medalia O
Sample:nuclear pore complex
Method:Subtomogram averaging (85 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: nuclear pore complex
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinnuclear pore complexfalseDictyostelium discoideum
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL50 mM Tris pH 7.6, 25 mM KCl, 5mM MgCl2, 50 mM sucrose
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER msVitrification instrument: Rudolf Gatz plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM300FEG/T300 kVFLOOD BEAMBRIGHT FIELD2.0 mm nm nm17500FIELD EMISSION GUNGATAN MULTISCAN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°63°GIF20000-20 eV90 K K K mrad e/Å2
Processing
Protocol:weighted backprojection, tomogram matching
Software:TOM
Resolution by author:85 Å
Resolution method:FSC 0.5
Processing details:3D reconstruction by averaging of subtomograms. Average number of projections used in the 3D reconstructions: 267.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails