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Title:Coat protein fold and maturation transition of bacteriophage P22 seen at subnanometer resolutions.
Authors:W. Jiang, Z. Li, Z. Zhang, M.L. Baker, P.E. Jr Prevelige, W. Chiu
Sample:P22 Mature Phage
Aggregation state:Icosahedral (9.5 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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Sample
Sample name: P22 Mature Phage
Theoretical molecular weight of the sample: 18
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusP22 mature phage18P22 mature phage02.054.0.03.001the icosahedral protein shell
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
mg/mL400 mesh copper grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%90 KHOMEMADE PLUNGER msVitrification instrument: home-made plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 4000EX400 kVFLOOD BEAMBRIGHT FIELD5.6 mm1000 nm3000 nm40LAB6Kodak SO163 film mmby monitoring Thon rings at very close to focus

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
side entryGATAN LIQUID NITROGEN0°0° eV95 K95 K95 K mrad30 e/Å2
Processing
Software:SAVR
CTF correction:per micrograph
Resolution by author:9.5 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
1007 μm/pixel8linkscanned at 7micrometer then averaged to 14micrometer step sizeZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1FH6 rigid bodycorrelation coefficientfoldhunter
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