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Title:Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation.
Authors:M. Diaconu, U Kothe, F. Schluenzen, J. Harms, N. Fischer, H. Stark, M.V. Rodnina, M.C. Wahl
Sample:E coli ribosome in complex with elongation factor G
Aggregation state:Single particle (18 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: E coli ribosome in complex with elongation factor G
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1ribosome-prokaryote70S
2proteinElongation Factor Gfalseunidentified
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.54.0 mg/mL50 mM Tris HCl, 70 mM NH4Cl, 30 mM KCl, 7 mM MgCl2, 1 mM DTT
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE40%93 KHOMEMADE PLUNGERBlotted manually for two seconds before plunging msVitrification instrument: home made
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG200 kVFLOOD BEAMBRIGHT FIELD2 mm1200 nm2000 nmFIELD EMISSION GUNKodak SO163 mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV93 K K K mrad15 e/Å2
Processing
Software:IMAGIC
CTF correction:Each particle
Resolution by author:18 Å
Resolution method:FSC at 5 sigma cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
124.0 μm/pixel1.38linkPRIMESCAN
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
Rigid BodyAmira 3.1, TGS SoftwareManual fitting
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