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Title:A quasi-atomic model of human adenovirus type 5 capsid.
Authors:Celine MS Fabry, Manuel Rosa-Calatrava, James Conway, Chloe Zubieta, Stephen Cusack, Rob WH Ruigrok, Guy Schoehn
Sample:Human adenovirus type 5
Aggregation state:Icosahedral (10.3 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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bullet_white.gif  Downloads
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Sample
Sample name: Human adenovirus type 5
Theoretical molecular weight of the sample: 150.0
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusunidentified adenovirus150unidentified adenovirus
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.53.0 mg/mL150 mM NaCl 20 mM Tris-HCl pH 7.5Cryo EMQuantifoil grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE%87 KZEISS PLUNGE FREEZER CRYOBOXManual blot for 1 second before plunging msVitrification instrument: Zeiss plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200T200 kVOTHERBRIGHT FIELD2 mm100 nm250 nm2750028600LAB6Kodak SO163 film mmobjective lens astigmatism was corrected at 100,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV90 K90 K90 K mrad10 e/Å22003-10-01
Processing
Software:PFT EM3DR
CTF correction:CTFMIX
Resolution by author:10.3 Å
Resolution method:FSC at 0.3 cut-off
Processing details:Final maps were calculated from 4100 single images
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
217 μm/pixel1.28linkZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
Rigid BodyR-factorURO SITUSRECIPROCALPenton bases were fitted using SITUS Hexons from the asymetric unit were fitted togheter using URO
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