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Title:Conservation of the capsid structure in tailed dsDNA bacteriophages: the pseudoatomic structure of phi29.
Authors:M.C. Morais, K.H. Choi, J.S. Koti, P.R. Chipman, DL. Anderson, M.G. Rossmann Chipman, D.L. Anderson, M.G. Rossmann
Sample:phi29 fiberless prohead particle
Aggregation state:Single particle (12.7 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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Sample
Sample name: phi29 fiberless prohead particle
Oligomeric state: capsid protein forms t3 q5 prolate icosahedron
Theoretical molecular weight of the sample: 12.4
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusbacteriophage phi2912.4bacteriophage phi29
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.8 mg/mL50 mM Tris-HCl pH7.8 10 mM MgCl2 100 mM NaClholey carbon
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM300FEG/T300 kVFLOOD BEAMBRIGHT FIELD2.0 mm2185 nm3781 nm33000FIELD EMISSION GUNKodak SO163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN9.0°9.0° eV K K K mrad20 e/Å2
Processing
Software:EMAN
CTF correction:CTF correction included phase and amplitude correction for each micrograph
Resolution by author:12.7 Å
Resolution method:FSC at 0.5
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
197 μm/pixel8linkafter scanning, images binned by a factor of 2ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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