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Title:Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly.
Authors:Wang HW, Nogales E
Sample:GMPCPP-tubulin
Aggregation state:Helical (18 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: GMPCPP-tubulin
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteintubulin0.11dimerfalse
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
6.82.0 mg/mL80 mM PIPES, 1 mM EGTA, 0.5 mM GMPCPP, 20 mM MgCl2400 mesh Quantifoil grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%90 KFEI VITROBOTBlot for 1.8 seconds before plunging with the Vitrobot offset set as -2 mm. msVitrification instrument: Vitrobot
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG200 kVFLOOD BEAMBRIGHT FIELD2.2 mm800 nm2000 nm3800038000FIELD EMISSION GUNKodak SO163 film mmobjective lens astigmatism was corected at 200K mag

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV90 K K K mrad15 e/Å22004-12-23
Processing
Software:Phoelix and MRC
CTF correction:each image
Resolution by author:18 Å
Resolution method:layer line with signal-to-noise ratio higher than 2.0
Processing details:Final map was calculated from two tube images
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
4012.7 μm/pixel14linkOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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