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Title:The structure of the poliovirus 135S cell entry intermediate at 10-angstrom resolution reveals the location of an externalized polypeptide that binds to membranes.
Authors:Bubeck D, Filman DJ, Cheng N, Steven AC, Hogle JM, Belnap DM
Sample:Poliovirus 135S particle
Aggregation state:Icosahedral (9.6 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: Poliovirus 135S particle
Oligomeric state: icosahedrally ordered capsid, 60 copies of VP1, VP2, VP3
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusHuman poliovirus 1, Mahoney strainHuman poliovirus 1, Mahoney strain00.052.0.01.007135S particle
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.5 mg/mL20 mM Tris, 2 mM CaCl2
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KBlotted manually before plunging msVitrification carried out in ambient atmosphere. Ethane cooled by liquid nitrogen.
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG120 kVFLOOD BEAMBRIGHT FIELD2 mm nm nm3800038500FIELD EMISSION GUNKodak SO-163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry liquid nitrogen-cooled cryo specimen holderGATAN LIQUID NITROGEN0°0° eV K K K mrad10 e/Å2
Processing
Software:EM3DR2
CTF correction:CTF and decay correction of each particle
Resolution by author:9.6 Å
Resolution method:FSC at 0.3 cut-off
Processing details:Reconstructed computed from focal pairs. Pairs not summed for reconstruction calculaton.
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
67 μm/pixel8linkDefocal pairs were used. Here, corresponding particle images from each micrograph are counted as one. Three focal pairs were scanned.ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
rigid bodyRECIPROCALsee paper for details of the model fitting
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