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Title:The structure of the poliovirus 135S cell entry intermediate at 10-angstrom resolution reveals the location of an externalized polypeptide that binds to membranes.
Authors:Bubeck D, Filman DJ, Cheng N, Steven AC, Hogle
Sample:Poliovirus 135S particle
Aggregation state:Icosahedral (10.3 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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Sample
Sample name: Poliovirus 135S particle
Oligomeric state: icosahedrally ordered capsid, 60 copies of VP1, VP2, VP3
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusHuman poliovirus 1, Mahoney strain.Complete virus identifier:ICTVdb 00.052.0.01.007Human poliovirus 1, Mahoney strain.Complete virus identifier:ICTVdb 00.052.0.01.00700.052.0.01.007135S particle
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.5 mg/mL20 mM HEPES, 2 mM CaCl2, 0.5 M NaCl
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KBlotted manually before plunging msVitrification carried out in ambient atmosphere. Ethane cooled by liquid nitrogen.
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG120 kVFLOOD BEAMBRIGHT FIELD2.0 mm nm nm3800038500FIELD EMISSION GUNKodak SO-163 film mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry, liquid nitrogen-cooled, cryo specimen holderGATAN LIQUID NITROGEN0°0° eV K K K mrad10 e/Å2
Processing
Software:EM3DR2
CTF correction:CTF and decay correction of each particle image
Resolution by author:10.3 Å
Resolution method:FSC at 0.3 cut-off
Processing details:Reconstructed computed from focal pairs. Pairs not summed for reconstruction calculaton.
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
47.0 μm/pixel8linkTwo defocal pairs were scanned.ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
rigid bodyRECIPROCALsee paper for details of the model fitting
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