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Title:The structure of the poliovirus 135S cell entry intermediate at 10-angstrom resolution reveals the location of an externalized polypeptide that binds to membranes.
Authors:D. Bubeck, D.J. Filman, N. Cheng, A.C. Steven, J.M. Hogle, D.M. Belnap
Sample:Poliovirus 135S particle
Aggregation state:Icosahedral (11 angstroms resolution)
Red flagLatest update:2011-05-26
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Sample
Sample name: Poliovirus 135S particle
Oligomeric state: icosahedrally ordered capsid, 60 copies of VP1, VP2, VP3
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusHuman poliovirus 1, Mahoney strainHuman poliovirus 1, Mahoney strain00.052.0.01.007135S particle
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.4 mg/mL20 mM HEPES, 2 mM CaCl2
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KPlunge frozen in liquid ethane. msVitrification occurred in ambient atmosphere.
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 20200 kVFLOOD BEAMBRIGHT FIELD mm nm nm50000FIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entry, liquid nitrogen-cooled, cryo specimen holderGATAN LIQUID NITROGEN0°0° eV K K K mrad10 e/Å2
Processing
Software:EM3DR2
CTF correction:CTF and decay correction of each particle image
Resolution by author:11 Å
Resolution method:FSC at 0.3 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
447.0 μm/pixel8link22 defocal pairs were scanned.ZEISS SCAI
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
rigid bodyRECIPROCALsee paper for details of the model fitting
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