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Title:Kelp fly virus: a novel group of insect picorna-like viruses as defined by genome sequence analysis and a distinctive virion structure.
Authors:C.J.Hartley, D.R.Greenwood, R.J.C.Gilbert,
Sample:Kelp fly virus
Aggregation state:Icosahedral (18.0 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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bullet_white.gif  Map information
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Sample
Sample name: Kelp fly virus
Oligomeric state: One icosahedral virion
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusKelp fly virusKelp fly virus
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.21.0 mg/mL100 mM TRIS, pH 7.2300 mesh copper grid, carbon lacey film
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KBlot until grid pulls away from Whatman number 1 paper before plunging ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI/PHILIPS CM200FEG200 kVSPOT SCANBRIGHT FIELD2 mm1000 nm7000 nm38000FIELD EMISSION GUNKodak SO163 film mmobjective lens astigmatism was corrected at 135,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV100 K K K mrad2 e/Å22001-06-01
Processing
Software:EMBL icosahedral
CTF correction:By micrograph
Resolution by author:18.0 Å
Resolution method:FSC at 0.5 cut-off
Processing details:Final map was calculated from images that had previously been CTF-corrected
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
274.38 μm/pixel8linkOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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