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Title:The structural basis for regulated assembly and function of the transcriptional activator NtrC.
Authors:De Carlo S., Chen B., Hoover T.R., Kondrashkina
Sample:Full length, active NtrC
Aggregation state:Single particle (28 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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bullet.png Experimental details
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Sample
Sample name: Full length, active NtrC
Oligomeric state: Hexamer
Theoretical molecular weight of the sample: 0.3
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinTranscription activator0.3HexamertrueSalmonella enterica subsp. enterica serovar TyphimuriumBaers following mutations: S160F, R456A, N457A, R461A
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
8.20.05 mg/mL200mM KCl, 20mM Tris, 1mM nucleotide, 0.2 mM BeCl2 and 5mM NaF, 5mM MgCl2, 5% Trehalose3% uranyl acetate
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% K ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 12120 kVFLOOD BEAMBRIGHT FIELD6.2 mm900 nm1500 nm5000049767LAB6Kodak SO163 mmcorrected

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
simple tiltOTHER0°50° eV K K K0 mrad20 e/Å2
Processing
Software:SPIDER
Resolution by author:28 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
186.7 μm/pixel14linkOTHER
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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