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Title:Cryo-EM asymmetric reconstruction of bacteriophage P22 reveals organization of its DNA packaging and infecting machinery.
Authors:J.Chang, P.Weigele, J.King, W.Chiu, W.Jiang
Sample:Bacteriophage P22
Aggregation state:Single particle (20 angstroms resolution)
Red flagLatest update:2011-05-26
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bullet_white.gif  Summary
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Sample
Sample name: Bacteriophage P22
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1virusEnterobacteria phage P220Enterobacteria phage P22
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.5 mg/mL10 mM Tris pH 7.5, 1mM MgCl2200 mesh copper grids
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100%297 KFEI VITROBOT msVitrification instrument: Vitrobot
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 2010F200 kVOTHERBRIGHT FIELD2 mm2000 nm5000 nm40000FIELD EMISSION GUNGatan mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Side entryGATAN LIQUID NITROGEN°° eV100 K K K mrad10 e/Å2
Processing
Software:EMAN
CTF correction:Each particle
Resolution by author:20 Å
Resolution method:FSC at 0.5 cut-off
Unit cell:
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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