top_cornerHomeEMD-1615  Contact EMDataBank 
Image unavailable
Title:Three-dimensional structure of YidC bound to the translating ribosome
Authors:Kohler R, Boehringer D, Greber B, Bingel-Erlenmeyer R, Collinson I, Schaffitzel C, Ban N
Sample:YidC bound to a translating ribosome
Method:Single particle reconstruction (14.4 angstroms resolution)
Red flagLatest update:2013-12-11
Sample
Sample name: YidC bound to a translating ribosome
Theoretical molecular weight of the sample: 2.6
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1ribosome-prokaryoteRibosome nascent chain complex2.6falseEscherichia coli
2proteinYidC0.062DimertrueEscherichia coli K-12GO:0051205IPR013308
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL200 mesh copper
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KHOMEMADE PLUNGER msVitrification instrument: Plunger
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 20200 kVFLOOD BEAMBRIGHT FIELD mm1000 nm4000 nm5000050000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN eV K K K mrad15 e/Å2
Processing
Protocol:Projection matching
Software:Imagic-5 Spider
CTF correction:Each image
Number of particles:24395
Resolution by author:14.4 Å
Resolution method:FSC at 0.5 (extrapolated to full the full dataset)
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
12.7 μm/pixel16NIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails