Home EMD-1615 Mirrors RCSB (USA) EBI (UK)  Contact us

Title: Three-dimensional structure of YidC bound to the translating ribosome Authors: Kohler R, Boehringer D, Greber B, Bingel-Erlenmeyer R, Collinson I, Schaffitzel C, Ban N Sample: YidC bound to a translating ribosome Method: Single particle reconstruction (14.4 angstroms resolution)   Summary  Experimental details   Visualization   Map information   Downloads Sample Sample name: YidC bound to a translating ribosome Theoretical molecular weight of the sample: 2.6 Components: ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details 1 ribosome-prokaryote Ribosome nascent chain complex 2.6 2 protein YidC 62 Dimer true Escherichia coli GO:0051205 IPR013308 Experiment Specimen state: Particle Specimen preparation: pH Specimen conc. Details Staining Specimen support details mg/mL 200 mesh copper Vitrification: Cryogen name Humidity Temp. Instr. Method Time resolved Details ETHANE % K HOMEMADE PLUNGER ms Vitrification instrument: Plunger Imaging: Microscope Voltage Illumination mode Imaging mode Cs Defocus min. Defocus max. Nominal mag. Calibrated mag. Electron source Detector Detector distance Astigmatism FEI TECNAI 20 200 kV FLOOD BEAM BRIGHT FIELD mm 1000 nm 4000 nm 50000 50000 FIELD EMISSION GUN Kodak SO163 film mm Specimen holder Holder model Tilt min. Tilt max. Energy filter Energy window Temp. Temp. min. Temp. max. Beam tilt Electron dose Other details Date Eucentric GATAN LIQUID NITROGEN 0° 0° eV K K K mrad 15 e/Å2 Processing Protocol: Projection matching Software: Imagic-5 Spider CTF correction: Each image Number of particles: 24395 Resolution by author: 14.4 Å Resolution method: FSC at 0.5 (extrapolated to full the full dataset) Scanned images: Num. images Sampling size OD range Quant. bit number Other details Scanner 12.7 μm/pixel 16 NIKON SUPER COOLSCAN 9000 Fitting: PDB Protocol Target crit. Software B value Fitting space PDB chain Details