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Title:Automatic Recovery of Missing Amplitudes and Phases in Tilt-Limited Electron Crystallography of 2D Crystals
Authors:Gipson B, Masiel DJ, Browning ND, Spence J, Mitsuoka K, Stahlberg H
Sample:Bacteriorhodopsin
Method:2D crystallography
Red flagLatest update:2012-11-07
Sample
Sample name: Bacteriorhodopsin
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinBacteriorhodopsinfalseHalobacterium salinarum
Experiment
Specimen state: 2D array
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL
Plane group:P 3
Unit cell:
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NITROGEN%4.2 KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL KYOTO-3000SFF300 kVOTHEROTHER mm nm nmOTHER mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
As per 1at9JEOL°° eV K K K mrad e/Å2The full details of the original dataset can be found from PDB entry 1at9
Processing
Protocol:PCO
Software:2dx (PCO Module)
CTF correction:Phase flipping and Electron Diffraction
Processing details:3D-PCO reconstructed maps, generated from phase-flipped raw 2D kimura, 97 crystallographic dataset.
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails