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Title: Automatic Recovery of Missing Amplitudes and Phases in Tilt-Limited Electron Crystallography of 2D Crystals Authors: Gipson B, Masiel DJ, Browning ND, Spence J, Mitsuoka K, Stahlberg H Sample: Bacteriorhodopsin Method: 2D crystallography Latest update: 2012-11-07   Summary  Experimental details   Visualization   Map information   Downloads Sample Sample name: Bacteriorhodopsin Components: ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details 1 protein Bacteriorhodopsin false Halobacterium salinarum Experiment Specimen state: 2D array Specimen preparation: pH Specimen conc. Details Staining Specimen support details mg/mL Plane group: P 3 Unit cell: Vitrification: Cryogen name Humidity Temp. Instr. Method Time resolved Details NITROGEN % 4.2 K NONE ms Imaging: Microscope Voltage Illumination mode Imaging mode Cs Defocus min. Defocus max. Nominal mag. Calibrated mag. Electron source Detector Detector distance Astigmatism JEOL KYOTO-3000SFF 300 kV OTHER OTHER mm nm nm OTHER mm Specimen holder Holder model Tilt min. Tilt max. Energy filter Energy window Temp. Temp. min. Temp. max. Beam tilt Electron dose Other details Date As per 1at9 JEOL ° ° eV K K K mrad e/Å2 The full details of the original dataset can be found from PDB entry 1at9 Processing Protocol: PCO Software: 2dx (PCO Module) CTF correction: Phase flipping and Electron Diffraction Processing details: 3D-PCO reconstructed maps, generated from phase-flipped raw 2D kimura, 97 crystallographic dataset. Fitting: PDB Protocol Target crit. Software B value Fitting space PDB chain Details