top_cornerHomeEMD-1902  Contact EMDataBank 
Image unavailable
Title:The HSV-1 UL17 protein is the second constituent of the capsid vertex specific component (CVSC) required for DNA packaging and retention.
Authors:Toropova K, Huffman JB, Homa FL, Conway JF
Sample:HSV-1 wild type C-capsid (KOS).
Method:Single particle reconstruction (16.5 angstroms resolution)
Red flagLatest update:2012-10-10
Sample
Sample name: HSV-1 wild type C-capsid (KOS).
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusHuman herpesvirus 1 strain KOSHuman herpesvirus 1 strain KOS
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL500 mM NaCl, 10 mM Tris, 1 mM EDTA
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE85% KFEI VITROBOT MARK III7.5 second blot before plunging msVitrification instrument: Vitrobot mark III
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm1630 nm2730 nm2900030000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV K K K mrad e/Å207-JUL-2010
Processing
Protocol:Polar Fourier Transform
Software:AUTO3DEM
CTF correction:Phase flip each particle
Number of particles:3973
Imposed symmetry:I
Resolution by author:16.5 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
486.35 μm/pixel8NIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails