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Title: Labeling and Localization of the Herpes Simplex Virus Capsid Protein UL25 and Its Interaction with the Two Triplexes Closest to the Penton. Authors: Conway JF, Cockrell SK, Copeland AM, Newcomb WW, Brown JC, Homa FL Sample: HSV-1 C-capsid with protein UL25 genetically labeled with GFP at amino acid 50. Method: Single particle reconstruction (13.7 angstroms resolution) Latest update: 2012-10-10   Summary  Experimental details   Visualization   Map information   Downloads Sample Sample name: HSV-1 C-capsid with protein UL25 genetically labeled with GFP at amino acid 50. Components: ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details 1 virus Human herpesvirus 1 strain KOS Human herpesvirus 1 strain KOS Experiment Specimen state: Particle Specimen preparation: pH Specimen conc. Details Staining Specimen support details 7.5 mg/mL 500 mM NaCl, 10 mM Tris, 1 mM EDTA Vitrification: Cryogen name Humidity Temp. Instr. Method Time resolved Details OTHER % K FEI VITROBOT MARK III ms Vitrification instrument: Vitrobot mark III. Cryogen was an equal mix of ethane-propane. Imaging: Microscope Voltage Illumination mode Imaging mode Cs Defocus min. Defocus max. Nominal mag. Calibrated mag. Electron source Detector Detector distance Astigmatism FEI TECNAI F20 200 kV FLOOD BEAM BRIGHT FIELD 2.0 mm nm nm 29000 30000 FIELD EMISSION GUN Kodak SO163 film mm Specimen holder Holder model Tilt min. Tilt max. Energy filter Energy window Temp. Temp. min. Temp. max. Beam tilt Electron dose Other details Date Eucentric GATAN LIQUID NITROGEN ° ° eV K K K mrad e/Å2 Processing Protocol: Polar Fourier Transform Software: AUTO3DEM CTF correction: Phase flip each particle Imposed symmetry: I Resolution by author: 13.7 Å Resolution method: FSC at 0.5 cut-off Scanned images: Num. images Sampling size OD range Quant. bit number Other details Scanner 6.35 μm/pixel 8 NIKON SUPER COOLSCAN 9000 Fitting: PDB Protocol Target crit. Software B value Fitting space PDB chain Details