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Title:Labeling and Localization of the Herpes Simplex Virus Capsid Protein UL25 and Its Interaction with the Two Triplexes Closest to the Penton.
Authors:Conway JF, Cockrell SK, Copeland AM, Newcomb WW, Brown JC, Homa FL
Sample:HSV-1 C-capsid with protein UL25 genetically labeled with GFP at amino acid 50.
Method:Single particle reconstruction (13.7 angstroms resolution)
Red flagLatest update:2012-10-10
Sample
Sample name: HSV-1 C-capsid with protein UL25 genetically labeled with GFP at amino acid 50.
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusHuman herpesvirus 1 strain KOSHuman herpesvirus 1 strain KOS
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL500 mM NaCl, 10 mM Tris, 1 mM EDTA
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
OTHER% KFEI VITROBOT MARK III msVitrification instrument: Vitrobot mark III. Cryogen was an equal mix of ethane-propane.
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm nm nm2900030000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV K K K mrad e/Å2
Processing
Protocol:Polar Fourier Transform
Software:AUTO3DEM
CTF correction:Phase flip each particle
Imposed symmetry:I
Resolution by author:13.7 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
6.35 μm/pixel8NIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails