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Title: Residues of the UL25 Protein of Herpes Simplex Virus That Are Required for Its Stable Interaction with Capsids. Authors: Cockrell SK, Huffman JB, Toropova K, Conway JF, Homa FL Sample: HSV-1 C-capsid with the UL25 protein labeled with a tandem affinity purification (TAP) tag at amino acid 50. Method: Single particle reconstruction (14.7 angstroms resolution) Latest update: 2012-10-10   Summary  Experimental details   Visualization   Map information   Downloads Sample Sample name: HSV-1 C-capsid with the UL25 protein labeled with a tandem affinity purification (TAP) tag at amino acid 50. Components: ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details 1 virus Human herpesvirus 1 strain KOS Human herpesvirus 1 strain KOS Experiment Specimen state: Particle Specimen preparation: pH Specimen conc. Details Staining Specimen support details 7.5 mg/mL 500 mM NaCl, 10 mM Tris, 1 mM EDTA Vitrification: Cryogen name Humidity Temp. Instr. Method Time resolved Details ETHANE 85% K FEI VITROBOT MARK III 6s blot prior to plunging ms Vitrification instrument: Vitrobot mark III Imaging: Microscope Voltage Illumination mode Imaging mode Cs Defocus min. Defocus max. Nominal mag. Calibrated mag. Electron source Detector Detector distance Astigmatism FEI TECNAI F20 200 kV FLOOD BEAM BRIGHT FIELD 2.0 mm 1250 nm 3730 nm 50000 50000 FIELD EMISSION GUN Kodak SO163 film mm Specimen holder Holder model Tilt min. Tilt max. Energy filter Energy window Temp. Temp. min. Temp. max. Beam tilt Electron dose Other details Date Eucentric GATAN LIQUID NITROGEN ° ° eV K K K mrad e/Å2 09-OCT-2009 Processing Protocol: Polar Fourier Transform Software: AUTO3DEM CTF correction: Phase flip each particle Number of particles: 4865 Imposed symmetry: I Resolution by author: 14.7 Å Resolution method: FSC at 0.5 cut-off Scanned images: Num. images Sampling size OD range Quant. bit number Other details Scanner 83 6.35 μm/pixel 8 NIKON SUPER COOLSCAN 9000 Fitting: PDB Protocol Target crit. Software B value Fitting space PDB chain Details