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Title:Residues of the UL25 Protein of Herpes Simplex Virus That Are Required for Its Stable Interaction with Capsids.
Authors:Cockrell SK, Huffman JB, Toropova K, Conway JF, Homa FL
Sample:HSV-1 C-capsid with the UL25 protein labeled with a tandem affinity purification (TAP) tag at amino acid 50.
Method:Single particle reconstruction (14.7 angstroms resolution)
Red flagLatest update:2012-10-10
Sample
Sample name: HSV-1 C-capsid with the UL25 protein labeled with a tandem affinity purification (TAP) tag at amino acid 50.
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1virusHuman herpesvirus 1 strain KOSHuman herpesvirus 1 strain KOS
Experiment
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL500 mM NaCl, 10 mM Tris, 1 mM EDTA
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE85% KFEI VITROBOT MARK III6s blot prior to plunging msVitrification instrument: Vitrobot mark III
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD2.0 mm1250 nm3730 nm5000050000FIELD EMISSION GUNKODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricGATAN LIQUID NITROGEN°° eV K K K mrad e/Å209-OCT-2009
Processing
Protocol:Polar Fourier Transform
Software:AUTO3DEM
CTF correction:Phase flip each particle
Number of particles:4865
Imposed symmetry:I
Resolution by author:14.7 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
836.35 μm/pixel8NIKON SUPER COOLSCAN 9000
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails