top_cornerHomeEMD-1951  Contact EMDataBank 
Image unavailable
Title:Structure of the CS1 pilus of enterotoxigenic Escherichia coli reveals structural polymorphism
Authors:Galkin VE, Kolappan S, Ng D, Zong Z, Li J, Yu X, Egelman EH, Craig L
Sample:CS1 pilus
Method:Helical reconstruction (20 angstroms resolution)
Red flagLatest update:2012-12-05
tab_top_blue.gif
bullet_white.gif  Summary
tab_bottom_blue.gif
pixel.gif
tab_top_white.gif
bullet.png Experimental details
tab_bottom_white.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Visualization
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Map information
tab_bottom_blue.gif
pixel.gif
tab_top_blue.gif
bullet_white.gif  Downloads
tab_bottom_blue.gif
pixel.gif
Sample
Sample name: CS1 pilus
Oligomeric state: Oligomer
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1cellular-componentCooA0.015OligomerfalseEscherichia coli
Experiment
Specimen state: Filament
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.40.2 mg/mL137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, 10 mM EDTA2% uranyl acetateglow discharged continuous carbon coated grids
Helical parameters:
HandPhi incrementAzimuthal (Z) increment
RIGHT HANDED113°8.7 Å
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 1280 kVOTHERBRIGHT FIELD2 mm1000 nm2000 nm3000030000LAB6Kodak SO163 film mmobjective lens astigmatism was corrected at 100,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
EucentricSIDE ENTRY, EUCENTRIC°° eV K K K mrad10 e/Å2Low dose mode
Processing
Protocol:Single particle reconstruction
Software:SPIDER, IHRSR
Resolution by author:20 Å
Resolution method:FSC at 0.5 cut-off
Processing details:Pilus segments were selected using the helixboxer program in SPIDER.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
104.16 μm/pixelNIKON COOLSCAN
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
3S0V rigid bodyREALProtocol: Rigid body. A single subunit was manually docked using CHIMERA and the filament was built by applying the symmetry parameters for the reconstruction.