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Title: Negative structure of open and closed conformation of crm1 Authors: Monecke T, Haselbach D, Neumann P, Thomson E, Hurt E, Stark H, Dickmanns A, Ficner R Sample: crm1 open conformation Method: Single particle reconstruction (18 angstroms resolution)   Summary  Experimental details   Visualization   Map information   Downloads Sample Sample name: crm1 open conformation Oligomeric state: monomer Theoretical molecular weight of the sample: 0.12 Components: ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details 1 protein crm1 monomer true Chaetomium thermophilum Experiment Specimen state: Particle Specimen preparation: pH Specimen conc. Details Staining Specimen support details 7.5 mg/mL 150 mM NaCl, 20 mM HEPES/NaOH pH 7.5, 2 mM MgCl2, 4 mM DTT Grids with adsorbed protein floated on 1% w/v uranyl formate for 60 seconds 200 mesh copper grid with thin carbon support, freshly floated on protein solution Vitrification: Cryogen name Humidity Temp. Instr. Method Time resolved Details NONE % K NONE ms Imaging: Microscope Voltage Illumination mode Imaging mode Cs Defocus min. Defocus max. Nominal mag. Calibrated mag. Electron source Detector Detector distance Astigmatism FEI/PHILIPS CM200FEG 160 kV SPOT SCAN BRIGHT FIELD mm nm nm 155000 FIELD EMISSION GUN TVIPS 4x4k CCD camera mm Specimen holder Holder model Tilt min. Tilt max. Energy filter Energy window Temp. Temp. min. Temp. max. Beam tilt Electron dose Other details Date OTHER ° ° eV 298 K K K mrad e/Å2 21-MAR-2012 Processing Protocol: Cross-common-lines Software: imagic CTF correction: each particle Number of particles: 21000 Resolution by author: 18 Å Resolution method: FSC at 0.5 cut-off Scanned images: Num. images Sampling size OD range Quant. bit number Other details Scanner 300 μm/pixel Fitting: PDB Protocol Target crit. Software B value Fitting space PDB chain Details