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Title: Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37 Authors: Skurnik M, Hyytiainen H, Happonen LJ, Kiljunen S, Datta N, Mattinen L, Williamson K, Kristo P, Szeliga M, Kalin-Manttari L, Ahola-Iivarinen E, Kalkkinen N, Butcher SJ Sample: Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37 Method: Single particle reconstruction (23.8 angstroms resolution) Latest update: 2012-10-24   Summary  Experimental details   Visualization   Map information   Downloads Sample Sample name: Electron cryo-microscopy of the intact (DNA-filled) head of the yersiniophage phiR1-37 Components: ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details 1 virus Yersinia phage phiR1-37 Yersinia phage phiR1-37 Experiment Specimen state: Particle Specimen preparation: pH Specimen conc. Details Staining Specimen support details mg/mL 50 mM Tris pH 7.8, 10 mM MgSO4 Vitrification: Cryogen name Humidity Temp. Instr. Method Time resolved Details ETHANE % K HOMEMADE PLUNGER ms Imaging: Microscope Voltage Illumination mode Imaging mode Cs Defocus min. Defocus max. Nominal mag. Calibrated mag. Electron source Detector Detector distance Astigmatism FEI TECNAI F20 200 kV FLOOD BEAM BRIGHT FIELD 2.0 mm 500 nm 5760 nm 62000 FIELD EMISSION GUN Kodak SO163 film mm Specimen holder Holder model Tilt min. Tilt max. Energy filter Energy window Temp. Temp. min. Temp. max. Beam tilt Electron dose Other details Date Side entry liquid nitrogen-cooled cryo specimen holder GATAN LIQUID NITROGEN ° ° eV K K K mrad 15 e/Å2 Low dose conditions 07-SEP-2009 Processing Software: AUTO3DEM CTF correction: Each micrograph Number of particles: 1028 Imposed symmetry: I Resolution by author: 23.8 Å Resolution method: FSC at 0.5 cut-off Scanned images: Num. images Sampling size OD range Quant. bit number Other details Scanner 297 μm/pixel ZEISS SCAI Fitting: PDB Protocol Target crit. Software B value Fitting space PDB chain Details