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Title:Characterization of the insertase for beta-barrel proteins of the outer mitochondrial membrane. 3-D reconstruction of the TOB complex
Authors:Klein A, Israel L, Lackey SWK, Nargang FE, Imhof A, Baumeister W, Neupert W, Thomas DR
Sample:TOB complex mitochondrial outer membrane complex required for inserting beta-barrel proteins into the outer membrane.
Method:Single particle reconstruction (14.7 angstroms resolution)
Red flagLatest update:2013-07-17
Visualization
3D reconstruction of TOB complexes isolated using the 9xHis tag on the Tob37 subunit. The TOB complex is a hetero trimer containing one copy each of Tob37, Tob38 and Tob37. Total mol. Wgt. Is 140 kDa. In this sample there are three isoforms of Tob55 54.7, 54.1 and 50.7 kDa respectively. This affects the reconstruction.
Suggested contour level: 0.08
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