3D reconstruction of TOB complexes isolated using the 9xHis tag on the Tob37 subunit. The TOB complex is a hetero trimer containing one copy each of Tob37, Tob38 and Tob37. Total mol. Wgt. Is 140 kDa. In this sample there are three isoforms of Tob55 54.7, 54.1 and 50.7 kDa respectively. This affects the reconstruction.