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Title:3D structure of myosin filaments isolated from human heart muscles by negative stain electron microscopy and single particle image analysis.
Authors:AL-Khayat HA, Kensler RW, Squire JM, Marston SB, Morris EP
Sample:3D map of human cardiac myosin filament
Method:Subtomogram averaging (28 angstroms resolution)
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Sample
Sample name: 3D map of human cardiac myosin filament
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinmyosin filamentfalseHomo sapiens3D map of human cardiac myosin filament
Experiment
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI 20200 kVSPOT SCANOTHER mm nm nm29000FIELD EMISSION GUNTVIPS TEMCAM-F415 (4k x 4k) mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
OTHER°° eV K K K mrad100 e/Å215-MAY-2012
Processing
Protocol:single particle analysis
Software:imagic
Resolution by author:28 Å
Resolution method:FSC at 0.5 cut-off, 3sigma and half-bit threshold
Processing details:Average number of tilts used in the 3D reconstructions: 285.
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
23717 μm/pixel16
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1myo rigid bodypymol and vedaREALProtocol: rigid body