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Title: Cryo EM map of the Gaalphaq-PLCbeta3 complex. Authors: Dutta S, Lyon AM, Tesmer JJG, Skiniotis G Sample: Cryo EM reconstruction of the Galphaq-PLCbeta3 complex. Method: Single particle reconstruction (21 angstroms resolution)   Summary  Experimental details   Visualization   Map information   Downloads Sample Sample name: Cryo EM reconstruction of the Galphaq-PLCbeta3 complex. Oligomeric state: Dimer Theoretical molecular weight of the sample: 1.8 Components: ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details 1 protein G alpha q 0.044 monomer true Mus musculus GO:0007202 IPR000654 2 protein phospholipase C beta 3 0.139 monomer true Homo sapiens GO:0007186 IPR016280 Experiment Specimen state: Particle Specimen preparation: pH Specimen conc. Details Staining Specimen support details 8.0 1 mg/mL 20 mM HEPES pH 8, 200 mM NaCl, 2 mM DTT, 0.9 mM CaCl2, 50 microM GDP, 30 microM AlCl3, 10 mM NaF, 5 mM MgCl2 glow-discharged Quantifoil R2/200 mesh grid Vitrification: Cryogen name Humidity Temp. Instr. Method Time resolved Details ETHANE 100% K FEI VITROBOT MARK IV Blot for 2 seconds at 3 microliter sample. ms Vitrification carried out in liquid nitrogen atmosphere. Imaging: Microscope Voltage Illumination mode Imaging mode Cs Defocus min. Defocus max. Nominal mag. Calibrated mag. Electron source Detector Detector distance Astigmatism FEI TECNAI F20 120 kV FLOOD BEAM BRIGHT FIELD 2 mm 1500 nm 3500 nm 50000 71138 FIELD EMISSION GUN US4000 CCD mm Objective lens astigmatism was corrected at 135,000 times magnification. Specimen holder Holder model Tilt min. Tilt max. Energy filter Energy window Temp. Temp. min. Temp. max. Beam tilt Electron dose Other details Date Used two holders. OTHER ° ° eV 89 K 89 K 95 K mrad 20 e/Å2 20-OCT-2011 Processing Protocol: cross-common lines Software: EMAN1 CTF correction: each particle Number of particles: 12673 Resolution by author: 21 Å Resolution method: FSC at 0.5 cut-off Scanned images: Num. images Sampling size OD range Quant. bit number Other details Scanner 350 μm/pixel Fitting: PDB Protocol Target crit. Software B value Fitting space PDB chain Details 4GNK  REAL