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Title:Cryo EM map of the Gaalphaq-PLCbeta3 complex.
Authors:Dutta S, Lyon AM, Tesmer JJG, Skiniotis G
Sample:Cryo EM reconstruction of the Galphaq-PLCbeta3 complex.
Method:Single particle reconstruction (21 angstroms resolution)
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Sample name: Cryo EM reconstruction of the Galphaq-PLCbeta3 complex.
Oligomeric state: Dimer
Theoretical molecular weight of the sample: 1.8
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinG alpha q0.044monomertrueMus musculusGO:0007202IPR000654
2proteinphospholipase C beta 30.139monomertrueHomo sapiensGO:0007186IPR016280
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
8.01 mg/mL20 mM HEPES pH 8, 200 mM NaCl, 2 mM DTT, 0.9 mM CaCl2, 50 microM GDP, 30 microM AlCl3, 10 mM NaF, 5 mM MgCl2glow-discharged Quantifoil R2/200 mesh grid
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE100% KFEI VITROBOT MARK IVBlot for 2 seconds at 3 microliter sample. msVitrification carried out in liquid nitrogen atmosphere.
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20120 kVFLOOD BEAMBRIGHT FIELD2 mm1500 nm3500 nm5000071138FIELD EMISSION GUNGATAN ULTRASCAN 4000 (4k x 4k) mmObjective lens astigmatism was corrected at 135,000 times magnification.

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
Used two holders.OTHER°° eV89 K89 K95 K mrad20 e/Å220-OCT-2011
Protocol:cross-common lines
CTF correction:each particle
Number of particles:12673
Imposed symmetry:C1
Resolution by author:21 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
350 μm/pixel
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails