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Title:Negative stain EM structure of the HOPS tethering complex (conformation 1)
Authors:Broecker C, Kuhlee A, Gatsogiannis C, Balderhaar HJ, Hoenscher C, Engelbrecht-Vandre S, Ungermann C, Raunser S
Sample:vacuole protein sorting (HOPS) tethering complex containing Vps11, Vps16, Vps18, Vps33, Vp39, Vps41
Method:Single particle reconstruction (30.4 angstroms resolution)
Sample name: vacuole protein sorting (HOPS) tethering complex containing Vps11, Vps16, Vps18, Vps33, Vp39, Vps41
Oligomeric state: Monomer
Theoretical molecular weight of the sample: 0.7
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinvacuolar protein sorting-associated protein 41MonomerfalseSaccharomyces cerevisiaeP38959GO:0030897
2proteinvacuolar protein sorting-associated protein 39MonomerfalseSaccharomyces cerevisiaeQ07468GO:0030897
3proteinvacuolar protein sorting-associated protein 33MonomerfalseSaccharomyces cerevisiaeP20795GO:0030897
4proteinvacuolar protein sorting-associated protein 18MonomerfalseSaccharomyces cerevisiaeP27801GO:0030897
5proteinvacuolar protein sorting-associated protein 11MonomerfalseSaccharomyces cerevisiaeP12868GO:0030897
6proteinvacuolar protein sorting-associated protein 16MonomerfalseSaccharomyces cerevisiaeQ03308GO:0030897
Specimen state: Particle
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
7.5 mg/mL1 M NaCl, 50 mM Hepes/NaOH, 10% glycerolGrids with adsorbed Protein floated on 0.07% Uranyl Formate for 1-3h at 4C.glow discharged 400 mesh copper grids with thin carbon support
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
JEOL 1400120 kVFLOOD BEAMBRIGHT FIELD3.4 mm1.3 nm1.7 nm50000LAB6KODAK SO-163 FILM mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
JEOL°° eV K K K mrad4.5 e/Å207-FEB-2011
Protocol:Projection matching
Number of particles:2979
Imposed symmetry:C1
Resolution by author:30.4 Å
Resolution method:FSC 0.5
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
92010.5 μm/pixel16OTHER
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails