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Title: Helical reconstruction of HMPV matrix protein-lipid filaments Authors: Leyrat C, Renner M, Harlos K, Huiskonen JT, Grimes JM Sample: Human metapneumovirus matrix protein M bound to DOPC Method: Helical reconstruction (28 angstroms resolution) Latest update: 2016-02-17 Other Views:   Summary  Experimental details   Visualization   Map information   Downloads Sample Sample name: Human metapneumovirus matrix protein M bound to DOPC Oligomeric state: helical Components: ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details 1 protein human metapneumovirus matrix protein dimer true Human metapneumovirus Q91F56 GO:0019068, GO:0019031 IPR005056 matrix protein was mixed with DOPC at a final concentration of 0.4 mM 2 ligand 1,2-Dioleoyl-sn-glycero-3-phosphocholine false synthetic construct Experiment Specimen state: Filament Specimen preparation: pH Specimen conc. Details Staining Specimen support details 7.5 0.2 mg/mL 20 mM Tris, 650 mM NaCl, 1M NDSB-201 Grids with adsorbed protein-lipid mixtures floated on 2% w/v uranyl acetate for 30 seconds 300 mesh copper grid with formvar carbon film Helical parameters: Phi increment Azimuthal (Z) increment 56.5° 5.16 Å Vitrification: Cryogen name Humidity Temp. Instr. Method Time resolved Details NONE % K NONE ms Imaging: Microscope Voltage Illumination mode Imaging mode Cs Defocus min. Defocus max. Nominal mag. Calibrated mag. Electron source Detector Detector distance Astigmatism FEI TECNAI F30 200 kV FLOOD BEAM BRIGHT FIELD mm 720 nm 820 nm 39000 48387 FIELD EMISSION GUN GATAN ULTRASCAN 4000 (4k x 4k) mm Specimen holder Holder model Tilt min. Tilt max. Energy filter Energy window Temp. Temp. min. Temp. max. Beam tilt Electron dose Other details Date SIDE ENTRY, EUCENTRIC ° ° eV 295 K K K mrad e/Å2 16-JUL-2013 Processing Protocol: Helical reconstruction Software: Burnham-Brandeis, Helical, Package CTF correction: Each micrograph Resolution by author: 28 Å Resolution method: Last visible layer line Processing details: Final map was filtered to 28 angstrom resolution. The reconstruction was calculated using Burnham-Brandeis Helical Package. Scanned images: Num. images Sampling size OD range Quant. bit number Other details Scanner 1 15 μm/pixel 12 Fitting: PDB Protocol Target crit. Software B value Fitting space PDB chain Details