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EMD-2415
RCSB (USA)
EBI (UK)
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Title:
Helical reconstruction of HMPV matrix protein-lipid filaments
Authors:
Leyrat C, Renner M, Harlos K, Huiskonen JT, Grimes JM
Sample:
Human metapneumovirus matrix protein M bound to DOPC
Method:
Helical reconstruction (28 angstroms resolution)
Latest update:
2016-02-17
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Summary
Experimental details
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Map information
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Sample
Sample name:
Human metapneumovirus matrix protein M bound to DOPC
Oligomeric state:
helical
Components:
ID
Type
Name
Exp. MW (MDa)
Oligomeric details
Recombinant expression
Synthetic
Organism
UniProt identifier
GO identifier
InterPro identifier
Virus identifier
Details
1
protein
human metapneumovirus matrix protein
dimer
true
Human metapneumovirus
Q91F56
GO:0019068
,
GO:0019031
IPR005056
matrix protein was mixed with DOPC at a final concentration of 0.4 mM
2
ligand
1,2-Dioleoyl-sn-glycero-3-phosphocholine
false
synthetic construct
Experiment
Specimen state:
Filament
Specimen preparation:
pH
Specimen conc.
Details
Staining
Specimen support details
7.5
0.2 mg/mL
20 mM Tris, 650 mM NaCl, 1M NDSB-201
Grids with adsorbed protein-lipid mixtures floated on 2% w/v uranyl acetate for 30 seconds
300 mesh copper grid with formvar carbon film
Helical parameters:
Phi increment
Azimuthal (Z) increment
56.5°
5.16 Å
Vitrification:
Cryogen name
Humidity
Temp.
Instr.
Method
Time resolved
Details
NONE
%
K
NONE
ms
Imaging:
Microscope
Voltage
Illumination mode
Imaging mode
Cs
Defocus min.
Defocus max.
Nominal mag.
Calibrated mag.
Electron source
Detector
Detector distance
Astigmatism
FEI TECNAI F30
200 kV
FLOOD BEAM
BRIGHT FIELD
mm
720 nm
820 nm
39000
48387
FIELD EMISSION GUN
GATAN ULTRASCAN 4000 (4k x 4k)
mm
Specimen holder
Holder model
Tilt min.
Tilt max.
Energy filter
Energy window
Temp.
Temp. min.
Temp. max.
Beam tilt
Electron dose
Other details
Date
SIDE ENTRY, EUCENTRIC
°
°
eV
295 K
K
K
mrad
e/Å
2
16-JUL-2013
Processing
Protocol:
Helical reconstruction
Software:
Burnham-Brandeis, Helical, Package
CTF correction:
Each micrograph
Resolution by author:
28 Å
Resolution method:
Last visible layer line
Processing details:
Final map was filtered to 28 angstrom resolution. The reconstruction was calculated using Burnham-Brandeis Helical Package.
Scanned images:
Num. images
Sampling size
OD range
Quant. bit number
Other details
Scanner
1
15 μm/pixel
12
Fitting:
PDB
Protocol
Target crit.
Software
B value
Fitting space
PDB chain
Details