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Title:3D structure of the ATP-bound yeast Vps4pE233Q dodecamer
Authors:M.J.Landsberg,P.R.Vajjhala,R.Rothnagel,A.L.Munn,B.Hankamer
Sample:6xHis tagged yeast Vps4pE233Q
Aggregation state:Single particle (23 angstroms resolution)
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bullet_white.gif  Summary
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bullet.png Experimental details
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bullet_white.gif  Visualization
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Sample
Sample name: 6xHis tagged yeast Vps4pE233Q
Oligomeric state: The complex is a dodecamer
Theoretical molecular weight of the sample: 0.59
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism GO identifier InterPro identifier Virus identifier Details
1proteinVacuolar protein sorting 40.56Dodecamertrue
Experiment
Sample preparation:
pHSample conc.DetailsStainingSample support details
7.40.1 mg/mL0.1M potassium acetate, 5mM magnesium acetate, 20mM Hepes, 1mM ATPGrids with adsorbed protein washed with an aqueous solution of 2% (w/v) uranyl acetate for 30 seconds.400 mesh copper grid
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
NONE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCs:Defocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F30300 kVFLOOD BEAMBRIGHT FIELD mm600 nm1100 nm59000FIELD EMISSION GUNGatan USC 4000 (model 895) mmobjective lens astigmatism was corrected at 93,000 times magnification

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
single tiltOTHER°° eV K K K mrad60 e/Å2
Processing
Software:IMAGIC, XMIPP, EMAN
Resolution by author:23 Å
Resolution method:rmeasure 0.5 cutoff
Processing details:FSC at 0.5 cut-off estimates resolution at 18.3
Unit cell:
Scanned images:
Num. imagesSampling sizeOD rangeQuant. bit numberOther detailsScanner
170 μm/pixel8link
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
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