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Title:Reconstruction of ParM in the open state using cryo-EM
Authors:Galkin VE, Orlova A, Rivera C, Mullins RD, Egelman EH
Sample:ParM
Method:Helical reconstruction
Red flagLatest update:2014-04-02
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Sample
Sample name: ParM
Components:
ID Type Name Exp. MW (MDa) Oligomeric details Recombinant expression Synthetic Organism UniProt identifier GO identifier InterPro identifier Virus identifier Details
1proteinParMfalseunidentified
Experiment
Specimen state: Filament
Specimen preparation:
pHSpecimen conc.DetailsStainingSpecimen support details
mg/mL
Helical parameters:
HandPhi incrementAzimuthal (Z) increment
RIGHT HANDED165°24.2 Å
Vitrification:
Cryogen nameHumidityTemp.Instr.MethodTime resolvedDetails
ETHANE% KNONE ms
Imaging:
MicroscopeVoltageIllumination modeImaging modeCsDefocus min.Defocus max.Nominal mag.Calibrated mag.Electron sourceDetectorDetector distanceAstigmatism
FEI TECNAI F20200 kVFLOOD BEAMBRIGHT FIELD mm nm nmFIELD EMISSION GUN mm

Specimen holderHolder modelTilt min.Tilt max.Energy filterEnergy windowTemp.Temp. min.Temp. max.Beam tiltElectron doseOther detailsDate
626GATAN LIQUID NITROGEN°° eV K K K mrad e/Å2
Processing
Fitting:
PDBProtocolTarget crit.SoftwareB valueFitting spacePDB chainDetails
1MWK REAL